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msigdb c2 curated gene sets collection  (Broad Institute Inc)

 
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    Broad Institute Inc msigdb c2 curated gene sets collection
    TGIF2 enhances LUAD cell migration and EMT in vitro. ( A ) <t>GSEA</t> of cell adhesion molecules enriched in TGIF2-low-expressing patients (upper panel). GSEA of EMT genes enriched in TGIF2-high-expressing patients (lower panel). ( B ) qRT-PCR analysis of EMT-related genes, including Fibronectin , u-PA , Slug , MMP9 , E-cadherin , Snail , Vimentin , ZEB1 , and Twist , in the indicated cells. ( C ) Immunofluorescence analysis of E-cadherin and Vimentin in A549 and H1299 cells with TGIF2 knockdown. Scale bars, 20 μm. ( D ) Western blot results of E-cadherin, Vimentin, and Fibronectin expression in TGIF2-knockdown A549 and H1299 cells. ( E ) Western blot results for E-cadherin, Vimentin, and Fibronectin expression in stable H1299 cells. ( F ) Transwell assay of the migration ability of TGIF2 knockdown A549 and H1299 cells. Scale bars, 100 μm. ( G ) Wound healing assay of the migrated distance of TGIF2 knockdown A549 and H1299 cells. Scale bars, 100 μm. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, not significant. All data are representative of three repeated experiments
    Msigdb C2 Curated Gene Sets Collection, supplied by Broad Institute Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/msigdb c2 curated gene sets collection/product/Broad Institute Inc
    Average 90 stars, based on 1 article reviews
    msigdb c2 curated gene sets collection - by Bioz Stars, 2026-05
    90/100 stars

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    1) Product Images from "Phosphorylation of TGIF2 represents a therapeutic target that drives EMT and metastasis of lung adenocarcinoma"

    Article Title: Phosphorylation of TGIF2 represents a therapeutic target that drives EMT and metastasis of lung adenocarcinoma

    Journal: BMC Cancer

    doi: 10.1186/s12885-023-10535-9

    TGIF2 enhances LUAD cell migration and EMT in vitro. ( A ) GSEA of cell adhesion molecules enriched in TGIF2-low-expressing patients (upper panel). GSEA of EMT genes enriched in TGIF2-high-expressing patients (lower panel). ( B ) qRT-PCR analysis of EMT-related genes, including Fibronectin , u-PA , Slug , MMP9 , E-cadherin , Snail , Vimentin , ZEB1 , and Twist , in the indicated cells. ( C ) Immunofluorescence analysis of E-cadherin and Vimentin in A549 and H1299 cells with TGIF2 knockdown. Scale bars, 20 μm. ( D ) Western blot results of E-cadherin, Vimentin, and Fibronectin expression in TGIF2-knockdown A549 and H1299 cells. ( E ) Western blot results for E-cadherin, Vimentin, and Fibronectin expression in stable H1299 cells. ( F ) Transwell assay of the migration ability of TGIF2 knockdown A549 and H1299 cells. Scale bars, 100 μm. ( G ) Wound healing assay of the migrated distance of TGIF2 knockdown A549 and H1299 cells. Scale bars, 100 μm. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, not significant. All data are representative of three repeated experiments
    Figure Legend Snippet: TGIF2 enhances LUAD cell migration and EMT in vitro. ( A ) GSEA of cell adhesion molecules enriched in TGIF2-low-expressing patients (upper panel). GSEA of EMT genes enriched in TGIF2-high-expressing patients (lower panel). ( B ) qRT-PCR analysis of EMT-related genes, including Fibronectin , u-PA , Slug , MMP9 , E-cadherin , Snail , Vimentin , ZEB1 , and Twist , in the indicated cells. ( C ) Immunofluorescence analysis of E-cadherin and Vimentin in A549 and H1299 cells with TGIF2 knockdown. Scale bars, 20 μm. ( D ) Western blot results of E-cadherin, Vimentin, and Fibronectin expression in TGIF2-knockdown A549 and H1299 cells. ( E ) Western blot results for E-cadherin, Vimentin, and Fibronectin expression in stable H1299 cells. ( F ) Transwell assay of the migration ability of TGIF2 knockdown A549 and H1299 cells. Scale bars, 100 μm. ( G ) Wound healing assay of the migrated distance of TGIF2 knockdown A549 and H1299 cells. Scale bars, 100 μm. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, not significant. All data are representative of three repeated experiments

    Techniques Used: Migration, In Vitro, Expressing, Quantitative RT-PCR, Immunofluorescence, Knockdown, Western Blot, Transwell Assay, Wound Healing Assay



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    msigdb c2 curated gene sets collection  (Broad Institute Inc)
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    TGIF2 enhances LUAD cell migration and EMT in vitro. ( A ) <t>GSEA</t> of cell adhesion molecules enriched in TGIF2-low-expressing patients (upper panel). GSEA of EMT genes enriched in TGIF2-high-expressing patients (lower panel). ( B ) qRT-PCR analysis of EMT-related genes, including Fibronectin , u-PA , Slug , MMP9 , E-cadherin , Snail , Vimentin , ZEB1 , and Twist , in the indicated cells. ( C ) Immunofluorescence analysis of E-cadherin and Vimentin in A549 and H1299 cells with TGIF2 knockdown. Scale bars, 20 μm. ( D ) Western blot results of E-cadherin, Vimentin, and Fibronectin expression in TGIF2-knockdown A549 and H1299 cells. ( E ) Western blot results for E-cadherin, Vimentin, and Fibronectin expression in stable H1299 cells. ( F ) Transwell assay of the migration ability of TGIF2 knockdown A549 and H1299 cells. Scale bars, 100 μm. ( G ) Wound healing assay of the migrated distance of TGIF2 knockdown A549 and H1299 cells. Scale bars, 100 μm. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, not significant. All data are representative of three repeated experiments
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    molecular signatures database (msigdb) h (hallmark) and c2 (curated) collection of gene sets  (Broad Institute Inc)
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    TGIF2 enhances LUAD cell migration and EMT in vitro. ( A ) <t>GSEA</t> of cell adhesion molecules enriched in TGIF2-low-expressing patients (upper panel). GSEA of EMT genes enriched in TGIF2-high-expressing patients (lower panel). ( B ) qRT-PCR analysis of EMT-related genes, including Fibronectin , u-PA , Slug , MMP9 , E-cadherin , Snail , Vimentin , ZEB1 , and Twist , in the indicated cells. ( C ) Immunofluorescence analysis of E-cadherin and Vimentin in A549 and H1299 cells with TGIF2 knockdown. Scale bars, 20 μm. ( D ) Western blot results of E-cadherin, Vimentin, and Fibronectin expression in TGIF2-knockdown A549 and H1299 cells. ( E ) Western blot results for E-cadherin, Vimentin, and Fibronectin expression in stable H1299 cells. ( F ) Transwell assay of the migration ability of TGIF2 knockdown A549 and H1299 cells. Scale bars, 100 μm. ( G ) Wound healing assay of the migrated distance of TGIF2 knockdown A549 and H1299 cells. Scale bars, 100 μm. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, not significant. All data are representative of three repeated experiments
    Molecular Signatures Database (Msigdb) H (Hallmark) And C2 (Curated) Collection Of Gene Sets, supplied by Broad Institute Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    TGIF2 enhances LUAD cell migration and EMT in vitro. ( A ) GSEA of cell adhesion molecules enriched in TGIF2-low-expressing patients (upper panel). GSEA of EMT genes enriched in TGIF2-high-expressing patients (lower panel). ( B ) qRT-PCR analysis of EMT-related genes, including Fibronectin , u-PA , Slug , MMP9 , E-cadherin , Snail , Vimentin , ZEB1 , and Twist , in the indicated cells. ( C ) Immunofluorescence analysis of E-cadherin and Vimentin in A549 and H1299 cells with TGIF2 knockdown. Scale bars, 20 μm. ( D ) Western blot results of E-cadherin, Vimentin, and Fibronectin expression in TGIF2-knockdown A549 and H1299 cells. ( E ) Western blot results for E-cadherin, Vimentin, and Fibronectin expression in stable H1299 cells. ( F ) Transwell assay of the migration ability of TGIF2 knockdown A549 and H1299 cells. Scale bars, 100 μm. ( G ) Wound healing assay of the migrated distance of TGIF2 knockdown A549 and H1299 cells. Scale bars, 100 μm. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, not significant. All data are representative of three repeated experiments

    Journal: BMC Cancer

    Article Title: Phosphorylation of TGIF2 represents a therapeutic target that drives EMT and metastasis of lung adenocarcinoma

    doi: 10.1186/s12885-023-10535-9

    Figure Lengend Snippet: TGIF2 enhances LUAD cell migration and EMT in vitro. ( A ) GSEA of cell adhesion molecules enriched in TGIF2-low-expressing patients (upper panel). GSEA of EMT genes enriched in TGIF2-high-expressing patients (lower panel). ( B ) qRT-PCR analysis of EMT-related genes, including Fibronectin , u-PA , Slug , MMP9 , E-cadherin , Snail , Vimentin , ZEB1 , and Twist , in the indicated cells. ( C ) Immunofluorescence analysis of E-cadherin and Vimentin in A549 and H1299 cells with TGIF2 knockdown. Scale bars, 20 μm. ( D ) Western blot results of E-cadherin, Vimentin, and Fibronectin expression in TGIF2-knockdown A549 and H1299 cells. ( E ) Western blot results for E-cadherin, Vimentin, and Fibronectin expression in stable H1299 cells. ( F ) Transwell assay of the migration ability of TGIF2 knockdown A549 and H1299 cells. Scale bars, 100 μm. ( G ) Wound healing assay of the migrated distance of TGIF2 knockdown A549 and H1299 cells. Scale bars, 100 μm. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, not significant. All data are representative of three repeated experiments

    Article Snippet: Gene set enrichment analysis (GSEA) was performed according to the authors' guidelines published on the Broad Institute web pages ( http://www.broadinstitute.org/gsea/index.jsp ) with cell adhesion molecules signature and epithelial-mesenchymal transition gene set (from the MSigDB C2 curated gene sets collection) using Signal2Noise ratios.

    Techniques: Migration, In Vitro, Expressing, Quantitative RT-PCR, Immunofluorescence, Knockdown, Western Blot, Transwell Assay, Wound Healing Assay